用大孔树脂来分离和纯化苯乳酸

论文摘要

苯乳酸由乳酸菌生产,它是一种重要的保鲜剂。苯乳酸是一个重要的防腐剂以及可以延长食品的保质期。本论文对苯乳酸进行研究其分离和纯化方法,并且研究它跟树脂的静态吸附和动态吸附。6种树脂（301,315,335,345,HZD-9,HZ-2）用来考查它们的吸附量和解吸量。结果表示301树脂的效果最好,因此以它进行下面的研究。首先将发酵液在活性炭加热处理（温度为80℃）,活性炭用量为5%。然后将发酵液的苯乳酸吸附在301树脂再用盐酸来洗脱被吸附的苯乳酸；萃取之前将流出溶液进行pH调解再用乙酸乙酯萃取。最后,用旋转蒸发器浓缩萃取溶液使苯乳酸结晶。HPLC流动相为A（三氟乙酸0.05%/水）/B（三氟乙酸0.05%/甲醇）50：50（v/v）,流速为1ml/min。苯乳酸保留时间在5分钟左右。301树脂对苯乳酸的吸附过程比较符合Langmuir方程,关系系数R2为0.99,自由能（△G）为负值。吸附过程的解析率和产品的纯度分别为87.31%和95.07%。

论文目录

• Abstract
• 摘要
• Table of contents
• List of figures
• List of tables
• Chapter 1 Introduction
• 1.1 General Introduction
• 1.2 Situation of Study
• 1.3 Contents of Study
• 1.4 Literature Review
• 1.4.1 Overview of Phenyllactic Acid
• 1.4.2 Extraction Methods
• 1.4.3 Studies of Resins, Adsorption and Desorption
• Chapter 2 Materials and Methods
• 2.1 Materials
• 2.2 Methods
• 2.2.1 Detection and Measurement for Phenyllactic Acid
• 2.2.2 Standard Curve Setting
• 2.2.3 Fermented Solution Preparation
• 2.2.4 Resin Selection
• 2.2.5 HCI Concentration Test
• 2.2.6 Static Adsorption and Desorption
• 2.2.7 Dynamic Adsorption and Desorption
• 2.2.8 Extraction
• 2.2.9 Effect of Eluting Solution pH on Extraction and Crystallization Test
• Chapter 3 Results and Discussions
• 3.1 Resin Selection
• 3.2 HCI Concentration Test
• 3.3 Static Adsorption and Desorption
• 3.4 Dynamic Adsorption and Desorption
• 3.5 Effect of Ph on Extraction and Crystallization
• Chapter 4 Conclusions and Prospects
• 4.1 Conclusions
• 4.2 Prospects
• References
• Acknowledgements