本文主要研究内容
作者张倩（2019）在《麦长管蚜识别报警信息素EBF的嗅觉蛋白（SaveOBP3/7/9）表达定位》一文中研究指出：麦长管蚜Sitobion avenae Fabricius是小麦植株上重要的刺吸式害虫。嗅觉系统在蚜虫对周围环境的感知、寄主植物定位及产卵地选择等过程中发挥重要作用。外界气味分子穿过触角感器孔道进入淋巴液与气味结合蛋白(OBPs)结合,进而开启下游的信号传递。(E)-β-farnesene(EBF)是麦长管蚜等多种蚜虫报警信息素的主要活性成分,EBF的释放会引起周围蚜虫的忌避行为。与EBF强烈结合的麦长管蚜气味结合蛋白主要是Save OBP3/7/9。目前,上述蛋白在蚜虫触角上的表达位置(感器)尚不清楚。本研究利用扫描电镜对麦长管蚜触角感器超显微结构进行了细致观察;利用whole-mount原位杂交技术结合荧光定量技术观察了Save OBP3/7/9在麦长管蚜羽化后不同时间点的表达定位和表达差异;实现了对Save OBP3/7/9在麦长管蚜成蚜触角上亚细胞水平的表达定位。取得的主要结果如下:1.麦长管蚜成蚜触角感器发育过程的超显微结构观察和Save OBP3/7/9在羽化不同时间节点的表达差异。结果如下:1)成蚜触角感器包括原生感觉圈、次生感觉圈、Ⅰ型和Ⅱ型毛形感器、钟形感器和腔锥状感器等;2)成蚜触角感器的类型及分布相对一致,均分布于触角第3节靠近基部的2/3范围内。数量上,有翅成蚜次生感觉圈的数量(7-13个)则明显多于无翅成蚜(1-5个);3)初羽成蚜触角第3节的次生感觉圈和梗节的腔锥状感器均尚未发育完全,其他感器则形态完整。羽化2h后,麦长管蚜触角与EBF识别相关的次生感觉圈发育完全;4)Save OBP3的表达量在羽化后2h开始上升,这与次生感觉圈数量发育完全是同步的;Save OBP9在麦长管蚜羽化后保持在稳定的较高水平表达;羽化6h后,主要在有翅次生感觉圈表达的Save OBP7表达量上升,该基因表达量动态与次生感觉圈的发育具对应关系,推测其不但参与识别EBF且功能相对专一。2.Save OBP3/7/9基因克隆。根据本实验室获得的Save OBP3/7/9基因全长序列,以Langfang-1为实验种群进行基因克隆。与NCBI数据库中Save OBP3/7/9基因序列比对结果显示,Save OBP7/9序列比对结果完全一致,而Save OBP3可能存在两个拷贝,上述结果为设计并合成Save OBP3/7/9原位杂交探针提供了精准模板。3.SaveOBP3/7/9的触角表达定位。whole-mount原位杂交SaveOBP3/7/9在麦长管蚜有翅和无翅成蚜触角感器上的表达定位结果显示:1)有翅成蚜靶标基因表达部位及感器类型包括触角第6鞭节的Ⅱ型毛形感器、原生感觉圈上的大、小板形感器、第5鞭节原生感觉圈的板形感器、第3鞭节次生感觉圈的板形感器以及触角第3-5鞭节的Ⅰ型毛形感器;2)无翅成蚜靶标基因表达部位主要在触角鞭节。其中,OBP3和OBP9均在触角第6节的Ⅱ型毛形感器、原生感觉圈中的大、小板形感器、第5节原生感觉圈的板形感器、第3节次生感觉圈的板形感器以及触角第4节的Ⅰ型毛形感器上表达;OBP7在触角第6节的Ⅱ型毛形感器、原生感觉圈中的大、小板形感器、第5节的原生感觉圈的板形感器以及触角第4节的Ⅰ型毛形感器上表达。
Abstract
mai chang guan ya Sitobion avenae Fabriciusshi xiao mai zhi zhu shang chong yao de ci xi shi hai chong 。xiu jiao ji tong zai ya chong dui zhou wei huan jing de gan zhi 、ji zhu zhi wu ding wei ji chan luan de shua ze deng guo cheng zhong fa hui chong yao zuo yong 。wai jie qi wei fen zi chuan guo chu jiao gan qi kong dao jin ru lin ba ye yu qi wei jie ge dan bai (OBPs)jie ge ,jin er kai qi xia you de xin hao chuan di 。(E)-β-farnesene(EBF)shi mai chang guan ya deng duo chong ya chong bao jing xin xi su de zhu yao huo xing cheng fen ,EBFde shi fang hui yin qi zhou wei ya chong de ji bi hang wei 。yu EBFjiang lie jie ge de mai chang guan ya qi wei jie ge dan bai zhu yao shi Save OBP3/7/9。mu qian ,shang shu dan bai zai ya chong chu jiao shang de biao da wei zhi (gan qi )shang bu qing chu 。ben yan jiu li yong sao miao dian jing dui mai chang guan ya chu jiao gan qi chao xian wei jie gou jin hang le xi zhi guan cha ;li yong whole-mountyuan wei za jiao ji shu jie ge ying guang ding liang ji shu guan cha le Save OBP3/7/9zai mai chang guan ya yu hua hou bu tong shi jian dian de biao da ding wei he biao da cha yi ;shi xian le dui Save OBP3/7/9zai mai chang guan ya cheng ya chu jiao shang ya xi bao shui ping de biao da ding wei 。qu de de zhu yao jie guo ru xia :1.mai chang guan ya cheng ya chu jiao gan qi fa yo guo cheng de chao xian wei jie gou guan cha he Save OBP3/7/9zai yu hua bu tong shi jian jie dian de biao da cha yi 。jie guo ru xia :1)cheng ya chu jiao gan qi bao gua yuan sheng gan jiao juan 、ci sheng gan jiao juan 、Ⅰxing he Ⅱxing mao xing gan qi 、zhong xing gan qi he qiang zhui zhuang gan qi deng ;2)cheng ya chu jiao gan qi de lei xing ji fen bu xiang dui yi zhi ,jun fen bu yu chu jiao di 3jie kao jin ji bu de 2/3fan wei nei 。shu liang shang ,you chi cheng ya ci sheng gan jiao juan de shu liang (7-13ge )ze ming xian duo yu mo chi cheng ya (1-5ge );3)chu yu cheng ya chu jiao di 3jie de ci sheng gan jiao juan he geng jie de qiang zhui zhuang gan qi jun shang wei fa yo wan quan ,ji ta gan qi ze xing tai wan zheng 。yu hua 2hhou ,mai chang guan ya chu jiao yu EBFshi bie xiang guan de ci sheng gan jiao juan fa yo wan quan ;4)Save OBP3de biao da liang zai yu hua hou 2hkai shi shang sheng ,zhe yu ci sheng gan jiao juan shu liang fa yo wan quan shi tong bu de ;Save OBP9zai mai chang guan ya yu hua hou bao chi zai wen ding de jiao gao shui ping biao da ;yu hua 6hhou ,zhu yao zai you chi ci sheng gan jiao juan biao da de Save OBP7biao da liang shang sheng ,gai ji yin biao da liang dong tai yu ci sheng gan jiao juan de fa yo ju dui ying guan ji ,tui ce ji bu dan can yu shi bie EBFju gong neng xiang dui zhuan yi 。2.Save OBP3/7/9ji yin ke long 。gen ju ben shi yan shi huo de de Save OBP3/7/9ji yin quan chang xu lie ,yi Langfang-1wei shi yan chong qun jin hang ji yin ke long 。yu NCBIshu ju ku zhong Save OBP3/7/9ji yin xu lie bi dui jie guo xian shi ,Save OBP7/9xu lie bi dui jie guo wan quan yi zhi ,er Save OBP3ke neng cun zai liang ge kao bei ,shang shu jie guo wei she ji bing ge cheng Save OBP3/7/9yuan wei za jiao tan zhen di gong le jing zhun mo ban 。3.SaveOBP3/7/9de chu jiao biao da ding wei 。whole-mountyuan wei za jiao SaveOBP3/7/9zai mai chang guan ya you chi he mo chi cheng ya chu jiao gan qi shang de biao da ding wei jie guo xian shi :1)you chi cheng ya ba biao ji yin biao da bu wei ji gan qi lei xing bao gua chu jiao di 6bian jie de Ⅱxing mao xing gan qi 、yuan sheng gan jiao juan shang de da 、xiao ban xing gan qi 、di 5bian jie yuan sheng gan jiao juan de ban xing gan qi 、di 3bian jie ci sheng gan jiao juan de ban xing gan qi yi ji chu jiao di 3-5bian jie de Ⅰxing mao xing gan qi ;2)mo chi cheng ya ba biao ji yin biao da bu wei zhu yao zai chu jiao bian jie 。ji zhong ,OBP3he OBP9jun zai chu jiao di 6jie de Ⅱxing mao xing gan qi 、yuan sheng gan jiao juan zhong de da 、xiao ban xing gan qi 、di 5jie yuan sheng gan jiao juan de ban xing gan qi 、di 3jie ci sheng gan jiao juan de ban xing gan qi yi ji chu jiao di 4jie de Ⅰxing mao xing gan qi shang biao da ;OBP7zai chu jiao di 6jie de Ⅱxing mao xing gan qi 、yuan sheng gan jiao juan zhong de da 、xiao ban xing gan qi 、di 5jie de yuan sheng gan jiao juan de ban xing gan qi yi ji chu jiao di 4jie de Ⅰxing mao xing gan qi shang biao da 。
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论文详细介绍
论文作者分别是来自中国农业科学院的张倩，发表于刊物中国农业科学院2019-07-05论文，是一篇关于麦长管蚜论文,原位杂交论文,气味结合蛋白论文,触角感器论文,中国农业科学院2019-07-05论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自中国农业科学院2019-07-05论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：麦长管蚜论文; 原位杂交论文; 气味结合蛋白论文; 触角感器论文; 中国农业科学院2019-07-05论文;