本文主要研究内容
作者申艳婷（2019）在《水稻病原诱导元件AATCA及其互作转录因子的功能鉴定》一文中研究指出：水稻是世界上重要的粮食作物。由立枯丝核菌(Rhizoctonia solani Kühn)引起的水稻纹枯病是极具破坏性的水稻病害之一。目前水稻病害研究大多集中在白叶枯病和稻瘟病上,很少有针对纹枯病的相关研究。而对水稻纹枯病的抗病机制与调控机理的研究更是少之又少。当前生产上主要是通过培育种植抗病品种来防治作物病害。但由于水稻对纹枯病的抗性是数量性状,所以很难通过常规育种手段来产生高抗纹枯病品种。纹枯病高抗材料的缺乏限制了其抗性育种。因此研究调控抗病相关基因表达的启动子及其互作转录因子进行抗病育种是防治水稻纹枯病的有效策略。前期实验室研究发现,水稻基因Os2H16能够被纹枯病菌所诱导,超量表达Os2H16增强了水稻对纹枯病菌的抗性,抑制表达Os2H16会使水稻对纹枯病菌更加敏感。Os2H16基因启动子是能够被纹枯病菌诱导的病原诱导表达型启动子,并且已经鉴定到了该启动子的主要响应区域为-513 bp至-411 bp以及-411 bp至-309 bp。前期通过烟草瞬时表达系统研究发现一个新的存在于-513 bp至-411 bp区域的能够独立响应纹枯病菌的顺式元件AATCA。本研究通过将AATCA元件转入水稻中进行表达发现其可以响应纹枯病菌的诱导,验证了前期实验室在烟草瞬时表达中的结果。我们通过DNA pull-down技术筛选得到与AATCA元件互作的蛋白。随后运用酵母单杂交技术验证了AATCA元件与OsbHLH057蛋白的互作。通过对OsbHLH057基因进行分析发现其编码一个功能未知的蛋白,具有HLH保守结构域,且定位于细胞核。对OsbHLH057基因的病原诱导表达模式进行分析,发现其可以受纹枯病菌诱导表达,暗示它可能参与水稻对纹枯病菌的响应。然后我们还构建了OsbHLH057基因超量与基因编辑表达载体,获得了水稻超量植株和敲除突变体植株,并且检测了其下游抗病相关基因Os2H16的表达,发现在敲除突变体中该基因表达均有一定程度的下调,表明OsbHLH057基因可能通过影响Os2H16的表达来正调控水稻对纹枯病的抗性。终上所述,本研究对顺式作用元件AATCA及其互作转录因子OsbHLH057进行研究,为利用调控抗病基因的表达,来提高水稻纹枯病抗性的研究提供了新的理论基础,对进一步研究水稻对纹枯病的抗性机制以及培育抗性水稻品种具有重要的意义。
Abstract
shui dao shi shi jie shang chong yao de liang shi zuo wu 。you li ku si he jun (Rhizoctonia solani Kühn)yin qi de shui dao wen ku bing shi ji ju po huai xing de shui dao bing hai zhi yi 。mu qian shui dao bing hai yan jiu da duo ji zhong zai bai xie ku bing he dao wen bing shang ,hen shao you zhen dui wen ku bing de xiang guan yan jiu 。er dui shui dao wen ku bing de kang bing ji zhi yu diao kong ji li de yan jiu geng shi shao zhi you shao 。dang qian sheng chan shang zhu yao shi tong guo pei yo chong zhi kang bing pin chong lai fang zhi zuo wu bing hai 。dan you yu shui dao dui wen ku bing de kang xing shi shu liang xing zhuang ,suo yi hen nan tong guo chang gui yo chong shou duan lai chan sheng gao kang wen ku bing pin chong 。wen ku bing gao kang cai liao de que fa xian zhi le ji kang xing yo chong 。yin ci yan jiu diao kong kang bing xiang guan ji yin biao da de qi dong zi ji ji hu zuo zhuai lu yin zi jin hang kang bing yo chong shi fang zhi shui dao wen ku bing de you xiao ce lve 。qian ji shi yan shi yan jiu fa xian ,shui dao ji yin Os2H16neng gou bei wen ku bing jun suo you dao ,chao liang biao da Os2H16zeng jiang le shui dao dui wen ku bing jun de kang xing ,yi zhi biao da Os2H16hui shi shui dao dui wen ku bing jun geng jia min gan 。Os2H16ji yin qi dong zi shi neng gou bei wen ku bing jun you dao de bing yuan you dao biao da xing qi dong zi ,bing ju yi jing jian ding dao le gai qi dong zi de zhu yao xiang ying ou yu wei -513 bpzhi -411 bpyi ji -411 bpzhi -309 bp。qian ji tong guo yan cao shun shi biao da ji tong yan jiu fa xian yi ge xin de cun zai yu -513 bpzhi -411 bpou yu de neng gou du li xiang ying wen ku bing jun de shun shi yuan jian AATCA。ben yan jiu tong guo jiang AATCAyuan jian zhuai ru shui dao zhong jin hang biao da fa xian ji ke yi xiang ying wen ku bing jun de you dao ,yan zheng le qian ji shi yan shi zai yan cao shun shi biao da zhong de jie guo 。wo men tong guo DNA pull-downji shu shai shua de dao yu AATCAyuan jian hu zuo de dan bai 。sui hou yun yong jiao mu chan za jiao ji shu yan zheng le AATCAyuan jian yu OsbHLH057dan bai de hu zuo 。tong guo dui OsbHLH057ji yin jin hang fen xi fa xian ji bian ma yi ge gong neng wei zhi de dan bai ,ju you HLHbao shou jie gou yu ,ju ding wei yu xi bao he 。dui OsbHLH057ji yin de bing yuan you dao biao da mo shi jin hang fen xi ,fa xian ji ke yi shou wen ku bing jun you dao biao da ,an shi ta ke neng can yu shui dao dui wen ku bing jun de xiang ying 。ran hou wo men hai gou jian le OsbHLH057ji yin chao liang yu ji yin bian ji biao da zai ti ,huo de le shui dao chao liang zhi zhu he qiao chu tu bian ti zhi zhu ,bing ju jian ce le ji xia you kang bing xiang guan ji yin Os2H16de biao da ,fa xian zai qiao chu tu bian ti zhong gai ji yin biao da jun you yi ding cheng du de xia diao ,biao ming OsbHLH057ji yin ke neng tong guo ying xiang Os2H16de biao da lai zheng diao kong shui dao dui wen ku bing de kang xing 。zhong shang suo shu ,ben yan jiu dui shun shi zuo yong yuan jian AATCAji ji hu zuo zhuai lu yin zi OsbHLH057jin hang yan jiu ,wei li yong diao kong kang bing ji yin de biao da ,lai di gao shui dao wen ku bing kang xing de yan jiu di gong le xin de li lun ji chu ,dui jin yi bu yan jiu shui dao dui wen ku bing de kang xing ji zhi yi ji pei yo kang xing shui dao pin chong ju you chong yao de yi yi 。
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论文作者分别是来自山东农业大学的申艳婷，发表于刊物山东农业大学2019-07-05论文，是一篇关于水稻纹枯病论文,顺式作用元件论文,转录因子论文,山东农业大学2019-07-05论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自山东农业大学2019-07-05论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：水稻纹枯病论文; 顺式作用元件论文; 转录因子论文; 山东农业大学2019-07-05论文;