本文主要研究内容
作者赵亚卓,王鑫,冯佳佳,孙丹丹,王凤茹,董金皋（2019）在《PH-START1调控拟南芥发育的功能分析》一文中研究指出：为明确PH-START1(At2g28320)调控拟南芥生长发育的功能,利用Real-time PCR技术对PH-START1在不同生长时期拟南芥的不同器官中的表达情况进行了分析,明确了PH-START1的时空表达特性;通过创制PH-START1功能获得和缺失的转基因拟南芥并分析其表型,明确了PH-START1在调控拟南芥生长发育过程中的生物学功能。Real-time PCR结果表明:PH-START1在拟南芥的根、叶、花、角果中都有表达。在幼苗期的根中表达量最多,在成苗期根中表达量逐渐减少;莲座叶中第6片莲座叶表达量最多,然后随着发育时间的延长逐渐减少;在花的发育过程中,随着花发育时间延长,PH-START1的表达量逐渐增多;授粉后3～5 d的角果中PH-START1的表达量最高,然后表达量逐渐降低,到授粉后9 d角果中PH-START1的表达量又略有增加。花中PH-START1在雄蕊中的表达量最高,其次是花瓣、萼片,在雌蕊中表达量最少。对PH-START1功能获得(过表达,OE)和缺失(T-DNA插入,ph-start1)的转基因拟南芥表型进行观察,分析PH-START1在调控拟南芥生长发育中的功能,发现过表达PH-START1拟南芥营养生长受到明显的抑制,地上部株型矮小、茎细弱、叶片明显变小,地下部根系发育也受到明显的抑制。这说明PH-STARTI在拟南芥生长发育中起负调控作用,为阐明和促控植物生长发育提供了理论依据。
Abstract
wei ming que PH-START1(At2g28320)diao kong ni na gai sheng chang fa yo de gong neng ,li yong Real-time PCRji shu dui PH-START1zai bu tong sheng chang shi ji ni na gai de bu tong qi guan zhong de biao da qing kuang jin hang le fen xi ,ming que le PH-START1de shi kong biao da te xing ;tong guo chuang zhi PH-START1gong neng huo de he que shi de zhuai ji yin ni na gai bing fen xi ji biao xing ,ming que le PH-START1zai diao kong ni na gai sheng chang fa yo guo cheng zhong de sheng wu xue gong neng 。Real-time PCRjie guo biao ming :PH-START1zai ni na gai de gen 、xie 、hua 、jiao guo zhong dou you biao da 。zai you miao ji de gen zhong biao da liang zui duo ,zai cheng miao ji gen zhong biao da liang zhu jian jian shao ;lian zuo xie zhong di 6pian lian zuo xie biao da liang zui duo ,ran hou sui zhao fa yo shi jian de yan chang zhu jian jian shao ;zai hua de fa yo guo cheng zhong ,sui zhao hua fa yo shi jian yan chang ,PH-START1de biao da liang zhu jian zeng duo ;shou fen hou 3～5 dde jiao guo zhong PH-START1de biao da liang zui gao ,ran hou biao da liang zhu jian jiang di ,dao shou fen hou 9 djiao guo zhong PH-START1de biao da liang you lve you zeng jia 。hua zhong PH-START1zai xiong rui zhong de biao da liang zui gao ,ji ci shi hua ban 、e pian ,zai ci rui zhong biao da liang zui shao 。dui PH-START1gong neng huo de (guo biao da ,OE)he que shi (T-DNAcha ru ,ph-start1)de zhuai ji yin ni na gai biao xing jin hang guan cha ,fen xi PH-START1zai diao kong ni na gai sheng chang fa yo zhong de gong neng ,fa xian guo biao da PH-START1ni na gai ying yang sheng chang shou dao ming xian de yi zhi ,de shang bu zhu xing ai xiao 、jing xi ruo 、xie pian ming xian bian xiao ,de xia bu gen ji fa yo ye shou dao ming xian de yi zhi 。zhe shui ming PH-STARTIzai ni na gai sheng chang fa yo zhong qi fu diao kong zuo yong ,wei chan ming he cu kong zhi wu sheng chang fa yo di gong le li lun yi ju 。
论文参考文献
论文详细介绍
论文作者分别是来自华北农学报的赵亚卓,王鑫,冯佳佳,孙丹丹,王凤茹,董金皋，发表于刊物华北农学报2019年02期论文，是一篇关于拟南芥论文,生长发育论文,华北农学报2019年02期论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自华北农学报2019年02期论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：拟南芥论文; 生长发育论文; 华北农学报2019年02期论文;