本文主要研究内容
作者毛立霞（2019）在《大白菜根肿病抗性基因的精细定位与标记开发》一文中研究指出：根肿病是由根肿菌侵染芸薹属植物的根部引起的,被称为芸薹属植物的“癌症”。大白菜属于芸薹属植物,产量受根肿病的影响十分严重。近年来,大白菜根肿病发病面积急剧增加,造成了重大的经济损失。因此,抗性基因的挖掘和抗根肿病品种的培育在大白菜根肿病的研究中具有十分重要的意义。本研究以抗根肿病材料欧洲芜菁ECD01和感病DH系Y510-9作为研究亲本,构建F2群体以及F2:3家系,利用BSA-Seq对抗根肿病基因Crr5进行定位,开发与之紧密连锁的分子标记,用于大白菜分子辅助育种。主要得到以下结果:1.对感病材料YX55、Y510-9和抗病材料ECD01、14CR514M-3被根肿菌侵染的根毛及皮层进行细胞学鉴定。结果表明感病材料YX55的平均根毛侵染率比抗病材料ECD01、14CR514M-3的平均根毛侵染率高47.53%-50.02%,Y510-9的平均根毛侵染率比ECD01的高40.06%。从皮层侵染来看,从第6天就观察到感病材料YX55、Y510-9的皮层被侵染。2.对F2群体进行根肿病鉴定和抗性遗传分析,经卡方检验,分离比例符合3:1,表明该根肿病抗性基因是由单基因控制的。构建极端DNA混池,进行BSA-Seq分析,将根肿病抗性基因定位于A08染色体的0.4-2.2 Mb之间,并将该基因命名为Crr5。3.开发20对多态性标记及用课题组已有的大白菜A08染色体上的标记筛选了5对多态性标记,用JoinMap 4.0对标记进行连锁分析,将Crr5基因定位在标记KMM32-4和Bra154-4之间,遗传距离分别是0.1 cM和0.4 cM,物理位置在1.1-1.6 Mb。利用开发的最佳标记之一KMM32-4对8个BC2F2群体进行正确性验证,其一致率在91.11%-100%之间,平均一致率为96.78%,该标记初步用于分子标记辅助选择。4.在1.1-1.6 Mb有57个预测基因,其中BraA08000154基因编码产物包含Leucine-rich repeat结构域,可能参与抗病过程。对这个基因的相对表达量进行分析。结果表明,同一时期这个基因在抗病植株的表达量显著高于感病植株的表达量,进一步表明这个基因可能为根肿病抗病基因Crr5的候选基因。
Abstract
gen zhong bing shi you gen zhong jun qin ran yun tai shu zhi wu de gen bu yin qi de ,bei chen wei yun tai shu zhi wu de “ai zheng ”。da bai cai shu yu yun tai shu zhi wu ,chan liang shou gen zhong bing de ying xiang shi fen yan chong 。jin nian lai ,da bai cai gen zhong bing fa bing mian ji ji ju zeng jia ,zao cheng le chong da de jing ji sun shi 。yin ci ,kang xing ji yin de wa jue he kang gen zhong bing pin chong de pei yo zai da bai cai gen zhong bing de yan jiu zhong ju you shi fen chong yao de yi yi 。ben yan jiu yi kang gen zhong bing cai liao ou zhou wu jing ECD01he gan bing DHji Y510-9zuo wei yan jiu qin ben ,gou jian F2qun ti yi ji F2:3jia ji ,li yong BSA-Seqdui kang gen zhong bing ji yin Crr5jin hang ding wei ,kai fa yu zhi jin mi lian suo de fen zi biao ji ,yong yu da bai cai fen zi fu zhu yo chong 。zhu yao de dao yi xia jie guo :1.dui gan bing cai liao YX55、Y510-9he kang bing cai liao ECD01、14CR514M-3bei gen zhong jun qin ran de gen mao ji pi ceng jin hang xi bao xue jian ding 。jie guo biao ming gan bing cai liao YX55de ping jun gen mao qin ran lv bi kang bing cai liao ECD01、14CR514M-3de ping jun gen mao qin ran lv gao 47.53%-50.02%,Y510-9de ping jun gen mao qin ran lv bi ECD01de gao 40.06%。cong pi ceng qin ran lai kan ,cong di 6tian jiu guan cha dao gan bing cai liao YX55、Y510-9de pi ceng bei qin ran 。2.dui F2qun ti jin hang gen zhong bing jian ding he kang xing wei chuan fen xi ,jing ka fang jian yan ,fen li bi li fu ge 3:1,biao ming gai gen zhong bing kang xing ji yin shi you chan ji yin kong zhi de 。gou jian ji duan DNAhun chi ,jin hang BSA-Seqfen xi ,jiang gen zhong bing kang xing ji yin ding wei yu A08ran se ti de 0.4-2.2 Mbzhi jian ,bing jiang gai ji yin ming ming wei Crr5。3.kai fa 20dui duo tai xing biao ji ji yong ke ti zu yi you de da bai cai A08ran se ti shang de biao ji shai shua le 5dui duo tai xing biao ji ,yong JoinMap 4.0dui biao ji jin hang lian suo fen xi ,jiang Crr5ji yin ding wei zai biao ji KMM32-4he Bra154-4zhi jian ,wei chuan ju li fen bie shi 0.1 cMhe 0.4 cM,wu li wei zhi zai 1.1-1.6 Mb。li yong kai fa de zui jia biao ji zhi yi KMM32-4dui 8ge BC2F2qun ti jin hang zheng que xing yan zheng ,ji yi zhi lv zai 91.11%-100%zhi jian ,ping jun yi zhi lv wei 96.78%,gai biao ji chu bu yong yu fen zi biao ji fu zhu shua ze 。4.zai 1.1-1.6 Mbyou 57ge yu ce ji yin ,ji zhong BraA08000154ji yin bian ma chan wu bao han Leucine-rich repeatjie gou yu ,ke neng can yu kang bing guo cheng 。dui zhe ge ji yin de xiang dui biao da liang jin hang fen xi 。jie guo biao ming ,tong yi shi ji zhe ge ji yin zai kang bing zhi zhu de biao da liang xian zhe gao yu gan bing zhi zhu de biao da liang ,jin yi bu biao ming zhe ge ji yin ke neng wei gen zhong bing kang bing ji yin Crr5de hou shua ji yin 。
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论文作者分别是来自郑州大学的毛立霞，发表于刊物郑州大学2019-07-03论文，是一篇关于大白菜根肿病论文,抗根肿病基因论文,精细定位论文,郑州大学2019-07-03论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自郑州大学2019-07-03论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：大白菜根肿病论文; 抗根肿病基因论文; 精细定位论文; 郑州大学2019-07-03论文;