论文摘要
白色念珠菌作为医学上常见的条件致病真菌,有关其细胞壁多糖成分的生物活性研究报道甚少。本实验通过培养白色念珠菌,提取细胞壁主要多糖成分—β-葡聚糖,来研究三种性质的β-葡聚糖,即白色念珠菌碱溶性β-葡聚糖（Candida albicans basic β-glucan,CABBG）、白色念珠菌酸溶性β-葡聚糖（Candida albicans acidic β-glucan,CAABG）和白色念珠菌不溶性β-葡聚糖（Candida albicans insoluble β-glucan,CAIBG）对小鼠白细胞生成的影响。 实验中自制提取白色念珠菌β-葡聚糖,通过急性毒性学实验,摸索出三种性质β-葡聚糖的LD50值,观察毒性靶器官和摸索药效学给药剂量。雄性昆明种小鼠72只随机分为六组:空白对照组、阴性对照组、阳性对照组和实验组（CABBG、CAABG、CAIBG三组）。空白对照组不施加任何处理,其它五组每日腹腔注射环磷酰胺（cyclophosphamide,endoxan,cytoxan,CTX）100mg/kg·d-1,连续三天,造成白细胞减少模型。实验第4天起,阴性对照组小鼠1mg/kg·d-1。尾静脉注射灭菌注射用水6天,阳性对照组小鼠以30μg/kg·d-1尾静脉注射rhG-CSF6天,三个实验组分别静脉注射CABBG 2.5mg/kg·d-1、CAABG 6.0mg/kg·d-1和CAIBG 51.2mg/kg·d-16天。所有动物均于实验前及造模后的第1、3、5、7天静脉取血计数外周血白细胞总数和分类数;动物随机处死后计算脾脏、胸腺指数并进行组织病理学检查。 结果表明所有实验组β-葡聚糖均对CTX所致的白细胞减少有回升作用,不溶性β-葡聚糖组作用更为明显。白色念珠菌β-葡聚糖尤其是不溶性β-葡聚糖是一种很有前途的免疫调节剂,值得进一步研究。
论文目录
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