论文摘要
目的：研究精神应激后部分脑区内BDNF、CREB蛋白及mRNA表达的时序性变化，进一步探讨BDNF对精神应激的保护作用及其机制。 方法：采用旁观电击大鼠应激模型作为精神应激模型，以旷场实验、高架十字迷宫实验评估大鼠的焦虑行为，利用免疫组织化学、原位杂交技术，观察精神应激后海马CA1区、CA3区、齿状回、前额叶皮质、杏仁核中央核、伏隔核(壳区)、中脑导水管周围灰质、腹侧背盖区、下丘脑背内侧核等脑区内BDNF、CREB蛋白及mRNA不同时点的表达。 结果： 1．行为学评定结果：在旷场实验中，外周格数、中央格数、以及修饰数均是生理应激组低于精神应激组，精神应激组低于正常对照组，差异具有显著性；在高架十字迷宫实验中，开放臂次数以及开放臂时间也是生理应激组最低，精神应激组居中，正常对照组最高，差异具有显著性。 2．精神应激后即刻，以上各脑区内BDNF蛋白及mRNA表达均明显下调，灰度值明显高于对照组，差异具有极显著性；精神应激后0.5h、1h BDNF蛋白及mRNA表达开始恢复，但仍少于对照组，差
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标签：脑源性神经营养因子论文; 反应元件结合蛋白论文; 精神应激论文; 氟西汀论文; 羟色胺血清素论文; 免疫组织化学论文; 原位杂交论文;