本文主要研究内容
作者刘杰（2019）在《鹅细小病毒与鸭圆环病毒共感染增强致病性研究》一文中研究指出：鹅细小病毒(GPV)属细小病毒科(parvoviridae)、细小病毒属(parvovirus),主要引起小鹅瘟,近年来,其在鸭群中可引起鸭大舌短喙综合征。鸭圆环病毒(DuCV)感染可导致宿主免疫抑制,通常与其他病原体共同感染使疾病进展更加复杂。GPV与DuCV共感染在田间广泛存在,但目前对GPV与DuCV共感染对宿主的影响情况不明。为探究GPV和DuCV是否协同增强了致病性,本研究通过临床病例分离鉴定了GPV与DuCV;通过建立GPV与DuCV共感染的动物模型,以病毒学、病理学、分子生物学等方法进行了GPV与DuCV协同复制与协同致病研究。病毒分离鉴定结果显示,分离得到的GPV与DuCV均为当前田间流行株,并制备了GPV多克隆抗体和DuCV多克隆抗体用于实验检测。将GPV与DuCV腹腔接种于2日龄樱桃谷肉鸭,在感染后第10天、第20天和第30天进行剖杀。GPV与DuCV共感染鸭表现精神沉郁,生长发育迟缓,羽毛凌乱,体重增长显著缓慢。血涂片观察可见共感染组鸭血液中异嗜性粒细胞和淋巴细胞明显增多。对血清中各种酶活力指标进行检测,结果发现,感染前期共感染组谷丙转氨酶(ALT)水平显著高于单感染组和正常组,反映共感染GPV和DuCV对肝脏的损伤加重。共感染组的大体病变明显比单感染组严重,表现为肝脏出血坏死,脾脏萎缩苍白,胸腺、法氏囊严重萎缩,骨髓苍白。组织病理学变化表现为:免疫系统中,脾脏红髓和白髓边界消失,淋巴细胞稀疏,出血,异嗜性粒细胞浸润;胸腺淋巴细胞稀疏,胸腺小叶间大量异嗜性粒细胞浸润,胸腺小体崩解;法氏囊髓质淋巴细胞稀疏;骨髓造血细胞大量流失。消化系统中,十二指肠绒毛上皮坏死脱落;肝脏严重空泡变性并有炎性细胞浸润。泌尿系统中,肾脏出血,肾小管上皮细胞空泡变性、坏死脱落。通过qPCR对各个时间点不同组织内病毒载量进行检测,我们发现,感染初期共感染组的病毒载量显著低于单感染组,但随着病程的发展,单感染组的病毒载量下降,GPV和DuCV共感染动物体内两种病毒的载量明显升高,均显著高于单感染组。通过IHC检测GPV和DuCV在体内的分布,在肝脏、脾脏、肾脏、十二指肠、法氏囊内检测到阳性细胞。随着日龄的增加,共感染组阳性细胞数量及信号强度明显高于单感染组,但两种病毒分布定位基本一致。感染初期,两种病毒均定位在肝细胞核,接毒后第30天,肝细胞的胞浆和胞核均能检测到阳性信号。肾脏的髓袢处的肾小管可检测到阳性细胞。感染前期,十二指肠中的阳性细胞主要在肠隐窝处,后期存在于十二指肠肠绒毛的杯状细胞中。法氏囊中DuCV阳性细胞主要分布在髓质,GPV阳性细胞在皮质和髓质均可检测到。综上所述,GPV与DuCV可协同促进各自的复制、协同增强致病性。本研究从致病性和病毒复制两方面揭示了共感染的GPV与DuCV具有协同致病作用。本研究不仅对后续研究GPV与DuCV共感染机制明确了方向,更将为免疫抑制病或其它禽病共感染研究提供了科学依据。
Abstract
e xi xiao bing du (GPV)shu xi xiao bing du ke (parvoviridae)、xi xiao bing du shu (parvovirus),zhu yao yin qi xiao e wen ,jin nian lai ,ji zai ya qun zhong ke yin qi ya da she duan hui zeng ge zheng 。ya yuan huan bing du (DuCV)gan ran ke dao zhi su zhu mian yi yi zhi ,tong chang yu ji ta bing yuan ti gong tong gan ran shi ji bing jin zhan geng jia fu za 。GPVyu DuCVgong gan ran zai tian jian an fan cun zai ,dan mu qian dui GPVyu DuCVgong gan ran dui su zhu de ying xiang qing kuang bu ming 。wei tan jiu GPVhe DuCVshi fou xie tong zeng jiang le zhi bing xing ,ben yan jiu tong guo lin chuang bing li fen li jian ding le GPVyu DuCV;tong guo jian li GPVyu DuCVgong gan ran de dong wu mo xing ,yi bing du xue 、bing li xue 、fen zi sheng wu xue deng fang fa jin hang le GPVyu DuCVxie tong fu zhi yu xie tong zhi bing yan jiu 。bing du fen li jian ding jie guo xian shi ,fen li de dao de GPVyu DuCVjun wei dang qian tian jian liu hang zhu ,bing zhi bei le GPVduo ke long kang ti he DuCVduo ke long kang ti yong yu shi yan jian ce 。jiang GPVyu DuCVfu qiang jie chong yu 2ri ling ying tao gu rou ya ,zai gan ran hou di 10tian 、di 20tian he di 30tian jin hang pou sha 。GPVyu DuCVgong gan ran ya biao xian jing shen chen yu ,sheng chang fa yo chi huan ,yu mao ling luan ,ti chong zeng chang xian zhe huan man 。xie tu pian guan cha ke jian gong gan ran zu ya xie ye zhong yi shi xing li xi bao he lin ba xi bao ming xian zeng duo 。dui xie qing zhong ge chong mei huo li zhi biao jin hang jian ce ,jie guo fa xian ,gan ran qian ji gong gan ran zu gu bing zhuai an mei (ALT)shui ping xian zhe gao yu chan gan ran zu he zheng chang zu ,fan ying gong gan ran GPVhe DuCVdui gan zang de sun shang jia chong 。gong gan ran zu de da ti bing bian ming xian bi chan gan ran zu yan chong ,biao xian wei gan zang chu xie huai si ,pi zang wei su cang bai ,xiong xian 、fa shi nang yan chong wei su ,gu sui cang bai 。zu zhi bing li xue bian hua biao xian wei :mian yi ji tong zhong ,pi zang gong sui he bai sui bian jie xiao shi ,lin ba xi bao xi shu ,chu xie ,yi shi xing li xi bao jin run ;xiong xian lin ba xi bao xi shu ,xiong xian xiao xie jian da liang yi shi xing li xi bao jin run ,xiong xian xiao ti beng jie ;fa shi nang sui zhi lin ba xi bao xi shu ;gu sui zao xie xi bao da liang liu shi 。xiao hua ji tong zhong ,shi er zhi chang rong mao shang pi huai si tuo la ;gan zang yan chong kong pao bian xing bing you yan xing xi bao jin run 。bi niao ji tong zhong ,shen zang chu xie ,shen xiao guan shang pi xi bao kong pao bian xing 、huai si tuo la 。tong guo qPCRdui ge ge shi jian dian bu tong zu zhi nei bing du zai liang jin hang jian ce ,wo men fa xian ,gan ran chu ji gong gan ran zu de bing du zai liang xian zhe di yu chan gan ran zu ,dan sui zhao bing cheng de fa zhan ,chan gan ran zu de bing du zai liang xia jiang ,GPVhe DuCVgong gan ran dong wu ti nei liang chong bing du de zai liang ming xian sheng gao ,jun xian zhe gao yu chan gan ran zu 。tong guo IHCjian ce GPVhe DuCVzai ti nei de fen bu ,zai gan zang 、pi zang 、shen zang 、shi er zhi chang 、fa shi nang nei jian ce dao yang xing xi bao 。sui zhao ri ling de zeng jia ,gong gan ran zu yang xing xi bao shu liang ji xin hao jiang du ming xian gao yu chan gan ran zu ,dan liang chong bing du fen bu ding wei ji ben yi zhi 。gan ran chu ji ,liang chong bing du jun ding wei zai gan xi bao he ,jie du hou di 30tian ,gan xi bao de bao jiang he bao he jun neng jian ce dao yang xing xin hao 。shen zang de sui pan chu de shen xiao guan ke jian ce dao yang xing xi bao 。gan ran qian ji ,shi er zhi chang zhong de yang xing xi bao zhu yao zai chang yin wo chu ,hou ji cun zai yu shi er zhi chang chang rong mao de bei zhuang xi bao zhong 。fa shi nang zhong DuCVyang xing xi bao zhu yao fen bu zai sui zhi ,GPVyang xing xi bao zai pi zhi he sui zhi jun ke jian ce dao 。zeng shang suo shu ,GPVyu DuCVke xie tong cu jin ge zi de fu zhi 、xie tong zeng jiang zhi bing xing 。ben yan jiu cong zhi bing xing he bing du fu zhi liang fang mian jie shi le gong gan ran de GPVyu DuCVju you xie tong zhi bing zuo yong 。ben yan jiu bu jin dui hou xu yan jiu GPVyu DuCVgong gan ran ji zhi ming que le fang xiang ,geng jiang wei mian yi yi zhi bing huo ji ta qin bing gong gan ran yan jiu di gong le ke xue yi ju 。
论文参考文献
论文详细介绍
论文作者分别是来自山东农业大学的刘杰，发表于刊物山东农业大学2019-07-08论文，是一篇关于鸭圆环病毒论文,鹅细小病毒论文,共感染论文,病毒复制论文,协同致病论文,山东农业大学2019-07-08论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自山东农业大学2019-07-08论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：鸭圆环病毒论文; 鹅细小病毒论文; 共感染论文; 病毒复制论文; 协同致病论文; 山东农业大学2019-07-08论文;